Ponciano M.Halos, Maria Etheliori A. Mariano, Masatoshi Matsumura, and Tadadtsu Makahara
Institute of Biology, College of Science, University of the Philippines Diliman
Natural Sciences Research Institute, University of the Philippines Diliman
University of Tsukuba
doi.org/10.57043/transnastphl.1998.5872
Abstract
The consortium of microorganisms used consisted of Alcaligenes sp., Flavobacterium indologenes, and Bacillus spp. These were isolated from Pasig River and Manila Bay through streak plating technique. These isolates were identified based on biochemical tests and morphological observations. They were introduced to Natural Seawater (NSW) and Mineral Medium C (MMC) with oil. The crude oil layer in the inoculated treattnents gradually diminished after eight weeks of continuous shaking compared to the uninoculated control treatment. Qualitative analyses of extracts from the control and inoculated liquid media wth crude oil were performed using gas chromatography. The consortium was effective in degrading crude oil.