Ronald R. Matias, Joyce Irene V. del Rosario, and HPV Study Group
Research and Biotechnology Division, St. Luke’s Medical
Department of Molecular Epidemiology, Institute of Tropical Medicine Nagasaki University
http://doi.org/10.57043/transnastphl.1999.5748
Abstract
Infection of cervical epithelial cells with human papillomavirnses (HPV) is a major factor in cervical cancer development. Out of more than 70 HPV types identified, about 30 types have been found to infect the genital and cervical mucosa. This study aimed to detect HPV in cervical cancer patients and identify which HPV genotypes are prevalent among Filipino women. Cervical tissue biopsies were collected from patients with cervical malignancies from various hospitals. A broad spectrum of mucosal HPV types have detected by consensus primer-mediated polymerase chain reaction (PCR) using the degenerate form of the MY09/MY 11 primer pair which amplifies a ~450 base pair segment of the HPV major capsid gene. Of the 162 patients in the study, 137 (84.6%) tested positive for HPV. PCR products generated are cloned into pUC18 and sequenced in ABI PRISM 310 Genetic Analyzer. Homology search and phylogenetic analysis of the resulting sequences from 11 samples show close sequence relation to HPV type 6.