{"id":5872,"date":"2026-03-04T10:19:53","date_gmt":"2026-03-04T02:19:53","guid":{"rendered":"https:\/\/transactions.nast.ph\/?p=5872"},"modified":"2026-03-27T00:27:23","modified_gmt":"2026-03-26T16:27:23","slug":"biodegradation-of-crude-oil-using-microorganisms-from-pasig-and-manila-bay","status":"publish","type":"post","link":"https:\/\/transactions.nast.ph\/?p=5872","title":{"rendered":"Biodegradation of Crude Oil Using Microorganisms from Pasig and Manila Bay"},"content":{"rendered":"\n<p class=\"has-text-align-center\">Ponciano M.Halos, Maria Etheliori A. Mariano, Masatoshi Matsumura, and Tadadtsu Makahara<br>Institute of Biology, College of Science, University of the Philippines Diliman<br>Natural Sciences Research Institute, University of the Philippines Diliman<br>University of Tsukuba<\/p>\n\n\n\n<p class=\"has-text-align-center\"><a href=\"http:\/\/doi.org\/10.57043\/transnastphl.1998.5872\" data-type=\"link\" data-id=\"doi.org\/10.57043\/transnastphl.1998.5872\">doi.org\/10.57043\/transnastphl.1998.5872<\/a><\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-1 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p><strong>Abstract<\/strong><\/p>\n\n\n\n<p>The consortium of microorganisms used consisted of Alcaligenes sp., Flavobacterium indologenes, and Bacillus spp. These were isolated from Pasig River and Manila Bay through streak plating technique. These isolates were identified based on biochemical tests and morphological observations. They were introduced to Natural Seawater (NSW) and Mineral Medium C (MMC) with oil. The crude oil layer in the inoculated treattnents gradually diminished after eight weeks of continuous shaking compared to the uninoculated control treatment. Qualitative analyses of extracts from the control and inoculated liquid media wth crude oil were performed using gas chromatography. The consortium was effective in degrading crude oil.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<h5 class=\"wp-block-heading\">Keywords<\/h5>\n\n\n<div class=\"taxonomy-post_tag wp-block-post-terms\"><a href=\"https:\/\/transactions.nast.ph\/?tag=alcaligenes-sp\" rel=\"tag\">Alcaligenes sp.<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=bacillus-spp\" rel=\"tag\">Bacillus spp.<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=biodegradation\" rel=\"tag\">biodegradation<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=bioremediation\" rel=\"tag\">bioremediation<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=consortium\" rel=\"tag\">consortium<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=crude-oil\" rel=\"tag\">crude oil<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=flavobacterium-indologenes\" rel=\"tag\">Flavobacterium indologenes<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=manila-bay\" rel=\"tag\">Manila Bay<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=microorganisms\" rel=\"tag\">microorganisms<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=pasig-river\" rel=\"tag\">Pasig River<\/a><\/div><\/div>\n<\/div>\n\n\n\n<div data-wp-interactive=\"core\/file\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!state.hasPdfPreview\"  class=\"wp-block-file__embed\" data=\"https:\/\/transactions.nast.ph\/wp-content\/uploads\/2026\/03\/TNP-1998-20_19_Biodegradation-of-Crude-Oil-using-Microorganisms-from-Pasig-and-Manila-Bay.pdf\" type=\"application\/pdf\" style=\"width:100%;height:600px\" aria-label=\"Embed of TNP 1998 (20)_19_Biodegradation of Crude Oil using Microorganisms from Pasig and Manila Bay.\"><\/object><a id=\"wp-block-file--media-ad848dd1-c1e0-41cd-8297-5003d46940d8\" href=\"https:\/\/transactions.nast.ph\/wp-content\/uploads\/2026\/03\/TNP-1998-20_19_Biodegradation-of-Crude-Oil-using-Microorganisms-from-Pasig-and-Manila-Bay.pdf\">TNP 1998 (20)_19_Biodegradation of Crude Oil using Microorganisms from Pasig and Manila Bay<\/a><a href=\"https:\/\/transactions.nast.ph\/wp-content\/uploads\/2026\/03\/TNP-1998-20_19_Biodegradation-of-Crude-Oil-using-Microorganisms-from-Pasig-and-Manila-Bay.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-ad848dd1-c1e0-41cd-8297-5003d46940d8\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Ponciano M.Halos, Maria Etheliori A. Mariano, Masatoshi Matsumura, and Tadadtsu Makahara<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[831],"tags":[1024,1027,150,1025,1026,1028,1030,1029,1031,1032],"class_list":{"0":"post-5872","1":"post","2":"type-post","3":"status-publish","4":"format-standard","6":"category-1998-technical-papers","7":"tag-alcaligenes-sp","8":"tag-bacillus-spp","9":"tag-biodegradation","10":"tag-bioremediation","11":"tag-consortium","12":"tag-crude-oil","13":"tag-flavobacterium-indologenes","14":"tag-manila-bay","15":"tag-microorganisms","16":"tag-pasig-river","17":"czr-hentry"},"_links":{"self":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts\/5872"}],"collection":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5872"}],"version-history":[{"count":2,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts\/5872\/revisions"}],"predecessor-version":[{"id":6137,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts\/5872\/revisions\/6137"}],"wp:attachment":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5872"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=5872"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=5872"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}