{"id":5773,"date":"2026-02-20T04:37:48","date_gmt":"2026-02-19T20:37:48","guid":{"rendered":"https:\/\/transactions.nast.ph\/?p=5773"},"modified":"2026-03-26T14:55:45","modified_gmt":"2026-03-26T06:55:45","slug":"a-novel-8-kd-sulfur-rich-protein-in-soybean-glycine-max-cotyledonpurification-and-gene-cloning","status":"publish","type":"post","link":"https:\/\/transactions.nast.ph\/?p=5773","title":{"rendered":"A Novel 8 KD Sulfur-Rich Protein in Soybean (Glycine Max) Cotyledon:Purification and Gene Cloning"},"content":{"rendered":"\n<p class=\"has-text-align-center\">Ma. Jamela R. Revilleza, Alfred Galvez, Deanne C. Krenz,<br>Benito O. De Lumen, and Evelyn Mae T. Mendoza<br>Institute of Chemistry, University of the Philippines at Los Ba\u00f1os (UPLB)<br>Dept. of Nutritional Sciences, University of California, Berkeley<br>Institute of Plant Breeding, UPLB<\/p>\n\n\n\n<p class=\"has-text-align-center\"><a href=\"http:\/\/doi.org\/10.57043\/transnastphl.1999.5773\">http:\/\/doi.org\/10.57043\/transnastphl.1999.5773<\/a><\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-1 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p><strong>Abstract<\/strong><\/p>\n\n\n\n<p>An 8 kD methionine-rich protein (MRP) (2D-1) was purified through a combination of selective enrichment of the albumin fraction through heparin-Sep ha rose affinity chromatography, 2D-SDS-PAGE and radiolabeling of methionine-containing proteins with [1-14C] iodoacetate. The MRP contains 8.6% methionine, 8.6% cysteine, 11.4% lysine and high amounts of glutamic and aspartic acids. The N-terminus sequence facilitated the synthesis of degenerate oligonucleotide primers for use in Polymerase Chain Reaction (PCR) to amplify cDNA fragments specifically coding for the gene. The PCR product was then used to screen a cDNA library and analyze the DNA sequence which confirmed the high sulfur amino content of 17.2% Southern analysis revealed the presence of two copies of the gene in the genome. This work lays down the groundwork for cloning of the gene which actually encodes the 8 kD MRP and a methionine poor 2.5 kD peptide .<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<h5 class=\"wp-block-heading\">Keywords<\/h5>\n\n\n<div class=\"taxonomy-post_tag wp-block-post-terms\"><span class=\"wp-block-post-terms__prefix\"><br><\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=heparin-sepharose-chromatography\" rel=\"tag\">Heparin-Sepharose chromatography<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=methionine-rich-proteins-mrp\" rel=\"tag\">Methionine-rich proteins (MRP)<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=n-terminus-cdna-library\" rel=\"tag\">N-terminus; cDNA library<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=polymerase-chain-reaction-pcr\" rel=\"tag\">Polymerase Chain Reaction (PCR)<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=soybean-glycine-max\" rel=\"tag\">soybean (Glycine max)<\/a><\/div><\/div>\n<\/div>\n\n\n\n<div data-wp-interactive=\"core\/file\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!state.hasPdfPreview\"  class=\"wp-block-file__embed\" data=\"https:\/\/transactions.nast.ph\/wp-content\/uploads\/2026\/02\/TNP-1999-21_9_A-Novel-8-KD-Sulfur-Rich-Protein-in-Soybean-Glycine-Max-Cotyledon-Purification-and-Gene-Cloning.pdf\" type=\"application\/pdf\" style=\"width:100%;height:1090px\" aria-label=\"Embed of TNP 1999 (21)_9_A Novel 8 KD Sulfur-Rich Protein in Soybean (Glycine Max) Cotyledon Purification and Gene Cloning.\"><\/object><a id=\"wp-block-file--media-55ff2ba5-f582-4ae7-be22-f0b3b6f1c296\" href=\"https:\/\/transactions.nast.ph\/wp-content\/uploads\/2026\/02\/TNP-1999-21_9_A-Novel-8-KD-Sulfur-Rich-Protein-in-Soybean-Glycine-Max-Cotyledon-Purification-and-Gene-Cloning.pdf\">TNP 1999 (21)_9_A Novel 8 KD Sulfur-Rich Protein in Soybean (Glycine Max) Cotyledon Purification and Gene Cloning<\/a><a href=\"https:\/\/transactions.nast.ph\/wp-content\/uploads\/2026\/02\/TNP-1999-21_9_A-Novel-8-KD-Sulfur-Rich-Protein-in-Soybean-Glycine-Max-Cotyledon-Purification-and-Gene-Cloning.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-55ff2ba5-f582-4ae7-be22-f0b3b6f1c296\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Ma. Jamela R. Revilleza, Alfred Galvez, Deanne C. Krenz, Benito O. De Lumen, and Evelyn Mae T. Mendoza<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[819],"tags":[880,882,878,881,879],"class_list":{"0":"post-5773","1":"post","2":"type-post","3":"status-publish","4":"format-standard","6":"category-1999-technical-papers","7":"tag-heparin-sepharose-chromatography","8":"tag-methionine-rich-proteins-mrp","9":"tag-n-terminus-cdna-library","10":"tag-polymerase-chain-reaction-pcr","11":"tag-soybean-glycine-max","12":"czr-hentry"},"_links":{"self":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts\/5773"}],"collection":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5773"}],"version-history":[{"count":3,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts\/5773\/revisions"}],"predecessor-version":[{"id":6051,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts\/5773\/revisions\/6051"}],"wp:attachment":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5773"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=5773"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=5773"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}