{"id":5753,"date":"2026-02-19T21:15:43","date_gmt":"2026-02-19T13:15:43","guid":{"rendered":"https:\/\/transactions.nast.ph\/?p=5753"},"modified":"2026-03-26T15:34:38","modified_gmt":"2026-03-26T07:34:38","slug":"molecular-cloning-of-the-philippine-isolate-of-banana-bunchy-topvirus-bbtv","status":"publish","type":"post","link":"https:\/\/transactions.nast.ph\/?p=5753","title":{"rendered":"Molecular Cloning of the Philippine Isolate of Banana Bunchy TopVirus (BBTV)"},"content":{"rendered":"\n<p class=\"has-text-align-center\">Vermando M. Aquino, Hui Wang, Evelyn Mae T. Mendoza, and John S. Hu<br>Institute of Plant Breeding, College of Agriculture, University of the Philippines <br>Department of Plant Pathology, University of Hawaii<\/p>\n\n\n\n<p class=\"has-text-align-center\"><a href=\"http:\/\/doi.org\/10.57043\/transnastphl.1999.5753\" data-type=\"link\" data-id=\"doi.org\/10.57043\/transnastphl.1999.5753\">doi.org\/10.57043\/transnastphl.1999.5753<\/a><\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-1 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p><strong>Abstract<\/strong><\/p>\n\n\n\n<p>Coding sequences of three single-stranded DNA (ssDNA) components from the Philippine isolate of banana bunchy top virus (BBTV) were cloned. These three viral genomic components encode a replicase protein, a coat protein and movement protein. A polymerase chain reaction (PCR) method was utilized to amplify specific ss DNA<br>sequences using total nucleic acid extracts from banana leaf samples. Using plant transformation vector pBI 121, constructs were made containing each of these three viral genes<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<h5 class=\"wp-block-heading\">Keywords<\/h5>\n\n\n<div class=\"taxonomy-post_tag wp-block-post-terms\"><a href=\"https:\/\/transactions.nast.ph\/?tag=banana\" rel=\"tag\">banana<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=banana-bunchy-top-virus-bbtv\" rel=\"tag\">Banana bunchy top virus (BBTV)<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=molecular-cloning\" rel=\"tag\">molecular cloning<\/a><\/div><\/div>\n<\/div>\n\n\n\n<div data-wp-interactive=\"core\/file\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!state.hasPdfPreview\"  class=\"wp-block-file__embed\" data=\"https:\/\/transactions.nast.ph\/wp-content\/uploads\/2026\/02\/TNP-1999-21_18_Molecular-Cloning-of-the-Philippine-Isolate-of-Banana-Bunchy-Top-Virus-BBTV.pdf\" type=\"application\/pdf\" style=\"width:100%;height:1090px\" aria-label=\"Embed of TNP 1999 (21)_18_Molecular Cloning of the Philippine Isolate of Banana Bunchy Top Virus (BBTV).\"><\/object><a id=\"wp-block-file--media-2ae5330c-220d-486d-aee7-45d9afd72211\" href=\"https:\/\/transactions.nast.ph\/wp-content\/uploads\/2026\/02\/TNP-1999-21_18_Molecular-Cloning-of-the-Philippine-Isolate-of-Banana-Bunchy-Top-Virus-BBTV.pdf\">TNP 1999 (21)_18_Molecular Cloning of the Philippine Isolate of Banana Bunchy Top Virus (BBTV)<\/a><a href=\"https:\/\/transactions.nast.ph\/wp-content\/uploads\/2026\/02\/TNP-1999-21_18_Molecular-Cloning-of-the-Philippine-Isolate-of-Banana-Bunchy-Top-Virus-BBTV.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-2ae5330c-220d-486d-aee7-45d9afd72211\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Vermando M. Aquino, Hui Wang, Evelyn Mae T. Mendoza, and John S. Hu<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[819],"tags":[847,945,944],"class_list":{"0":"post-5753","1":"post","2":"type-post","3":"status-publish","4":"format-standard","6":"category-1999-technical-papers","7":"tag-banana","8":"tag-banana-bunchy-top-virus-bbtv","9":"tag-molecular-cloning","10":"czr-hentry"},"_links":{"self":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts\/5753"}],"collection":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5753"}],"version-history":[{"count":3,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts\/5753\/revisions"}],"predecessor-version":[{"id":6070,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts\/5753\/revisions\/6070"}],"wp:attachment":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5753"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=5753"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=5753"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}