{"id":5744,"date":"2026-02-19T21:12:29","date_gmt":"2026-02-19T13:12:29","guid":{"rendered":"https:\/\/transactions.nast.ph\/?p=5744"},"modified":"2026-03-26T15:36:53","modified_gmt":"2026-03-26T07:36:53","slug":"a-new-medium-for-isolation-culture-and-metronidazole-sensitivity-testing-for-helicobacter-pyroli-diagnostic-value-for-early-eradication-of-h-pylori-infection","status":"publish","type":"post","link":"https:\/\/transactions.nast.ph\/?p=5744","title":{"rendered":"A New Medium for Isolation, Culture and Metronidazole-Sensitivity Testing for Helicobacter pyroli: Diagnostic Value for Early Eradication of H. pylori Infection"},"content":{"rendered":"\n<p class=\"has-text-align-center\">Blanquita B. de Guzman, Liza P. Faustino, Ma. Corazon 8. Paredes and Filipinas F. Natividad<br>Research and Biotechnology Division, St. Luke&#8217;s Medical Center<\/p>\n\n\n\n<p class=\"has-text-align-center\"><a href=\"http:\/\/doi.org\/10.57043\/transnastphl.1999.5744\" data-type=\"link\" data-id=\"doi.org\/10.57043\/transnastphl.1999.5744\">doi.org\/10.57043\/transnastphl.1999.5744<\/a><\/p>\n\n\n\n<div class=\"wp-block-columns is-layout-flex wp-container-core-columns-is-layout-1 wp-block-columns-is-layout-flex\">\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:66.66%\">\n<p><strong>Abstract<\/strong><\/p>\n\n\n\n<p>In the Philippines, resistance to metronidazole has become a problem in the eradication of Helicobacter pylori, a bacterium known to be the cause for chronic gastritis and peptic ulcer disease. In this study, a new medium was designed to ensure optimum growth of the bacteria for antimicrobial susceptibility testing. This complex basal medium used Columbia Blood agar base, urea agar base plus supplements such as horse serum, polyvitex, and peptone. For culture, antibiotics such as vancomycin, polymixin B, and trimethoprim lactate were added.<\/p>\n\n\n\n<p>Since the medium does not contain whole blood but has urea agar base in its composition, it is suitable for isolating H. pylori as well as direct testing of urease activity. This medium was tested using the six local isolates of H. pylori (as previously reported by the RBD Microbiology Laboratory) plus H. pylori ATCC 49503 strain as reference and other gram-negative bacteria such as Proteus vulgaris ATCC 13315 and Klebsiella pneumoniae. Agar plates which contained this medium were inoculated with the different isolates and bacterial strains then incubated at 37\u00b0C in a microaerophilic environment (5% O2\u200b, 10% CO2, and 85% N2) for 7 days. Confirmation of H. pylori was done by Gram staining and biochemical tests for catalase, oxidase, and urease.<\/p>\n<\/div>\n\n\n\n<div class=\"wp-block-column is-layout-flow wp-block-column-is-layout-flow\" style=\"flex-basis:33.33%\">\n<h5 class=\"wp-block-heading\">Keywords<\/h5>\n\n\n<div class=\"taxonomy-post_tag wp-block-post-terms\"><a href=\"https:\/\/transactions.nast.ph\/?tag=catalase\" rel=\"tag\">catalase<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=helicobacter-pylori\" rel=\"tag\">Helicobacter pylori<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=metronidazole-resistant\" rel=\"tag\">metronidazole-resistant<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=microacrophilic\" rel=\"tag\">microacrophilic<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=oxidasc\" rel=\"tag\">oxidasc<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=polymixin-b\" rel=\"tag\">polymixin B<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=trimethoprim-lactate\" rel=\"tag\">trimethoprim lactate<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=urea-agar-base-vancomycin\" rel=\"tag\">urea agar base. vancomycin<\/a><span class=\"wp-block-post-terms__separator\">, <\/span><a href=\"https:\/\/transactions.nast.ph\/?tag=urease\" rel=\"tag\">urease<\/a><\/div><\/div>\n<\/div>\n\n\n\n<div data-wp-interactive=\"core\/file\" class=\"wp-block-file\"><object data-wp-bind--hidden=\"!state.hasPdfPreview\"  class=\"wp-block-file__embed\" data=\"https:\/\/transactions.nast.ph\/wp-content\/uploads\/2026\/02\/TNP-1999-21_22_A-New-Medium-for-Isolation-Culture-and-Metronidazole-Sensitivity-Testing-of-Helicobacter-Pylori.pdf\" type=\"application\/pdf\" style=\"width:100%;height:1090px\" aria-label=\"Embed of TNP 1999 (21)_22_A New Medium for Isolation, Culture and Metronidazole-Sensitivity Testing of Helicobacter Pylori.\"><\/object><a id=\"wp-block-file--media-ccf2efe8-ba18-491c-bc78-9b157e144cd2\" href=\"https:\/\/transactions.nast.ph\/wp-content\/uploads\/2026\/02\/TNP-1999-21_22_A-New-Medium-for-Isolation-Culture-and-Metronidazole-Sensitivity-Testing-of-Helicobacter-Pylori.pdf\">TNP 1999 (21)_22_A New Medium for Isolation, Culture and Metronidazole-Sensitivity Testing of Helicobacter Pylori<\/a><a href=\"https:\/\/transactions.nast.ph\/wp-content\/uploads\/2026\/02\/TNP-1999-21_22_A-New-Medium-for-Isolation-Culture-and-Metronidazole-Sensitivity-Testing-of-Helicobacter-Pylori.pdf\" class=\"wp-block-file__button wp-element-button\" download aria-describedby=\"wp-block-file--media-ccf2efe8-ba18-491c-bc78-9b157e144cd2\">Download<\/a><\/div>\n","protected":false},"excerpt":{"rendered":"<p>Blanquita B. de Guzman, Liza P. Faustino, Ma. Corazon 8. Paredes and Filipinas F. Natividad<\/p>\n","protected":false},"author":3,"featured_media":0,"comment_status":"closed","ping_status":"closed","sticky":false,"template":"","format":"standard","meta":{"footnotes":""},"categories":[819],"tags":[969,970,974,967,972,971,975,968,973],"class_list":{"0":"post-5744","1":"post","2":"type-post","3":"status-publish","4":"format-standard","6":"category-1999-technical-papers","7":"tag-catalase","8":"tag-helicobacter-pylori","9":"tag-metronidazole-resistant","10":"tag-microacrophilic","11":"tag-oxidasc","12":"tag-polymixin-b","13":"tag-trimethoprim-lactate","14":"tag-urea-agar-base-vancomycin","15":"tag-urease","16":"czr-hentry"},"_links":{"self":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts\/5744"}],"collection":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts"}],"about":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/types\/post"}],"author":[{"embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/users\/3"}],"replies":[{"embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Fcomments&post=5744"}],"version-history":[{"count":4,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts\/5744\/revisions"}],"predecessor-version":[{"id":6078,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=\/wp\/v2\/posts\/5744\/revisions\/6078"}],"wp:attachment":[{"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Fmedia&parent=5744"}],"wp:term":[{"taxonomy":"category","embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Fcategories&post=5744"},{"taxonomy":"post_tag","embeddable":true,"href":"https:\/\/transactions.nast.ph\/index.php?rest_route=%2Fwp%2Fv2%2Ftags&post=5744"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}