Shingo Inoue1, Ronald R. Matias1,3, Futoshi Hasebe4, Kouichi Morita4, Kazunori Oishi5, Jhoe A. R. Alfon1, Jingle
R. Candelario1, Deo J.M. Cruz1, Giselle Espiritu1, Alma Gonzales1, Mary A. Pancho1, Cynthia Mapua1, Efren M.
Dimaano6, Celia Carlos2, Akira Igarashi4, and Filipinas F. Nativinad1
1 Research and Biotechnology Division,
2 Department of Pediatrics, St. Luke\u2019s Medical Center, Quezon City, Philippines
3 Institute of Biology, University of the Philippines, Diliman, Quezon City, Philippines
4 Department of Virology,
5 Department of Internal Medicine, Institute of Tropical Medicine, Nagasaki University, Nagasaki, Japan
6 San Lazaro Hospital, Pavilion1, Manila, Philippines<\/p>\n\n\n\n
http:\/\/doi.org\/10.57043\/transnastphl.2000.5404<\/a><\/p>\n\n\n\n Abstract<\/strong><\/p>\n\n\n\n Due to the similar symptoms with dengue fever (DF) and dengue hemorrhagic fever (DHF), Chikungunya (CHIK) virus infections were diagnosed as DF or DHF. In this study, we examined more than 300 serum samples collected from dengue-suspected patients in San Lazaro Hospital and St. Luke’s Medical Center. Reverse transcriptase-polymerase chain reaction (RT-PCR) and antigen sandwich enzyme-linked immunosorbent assay (ELISA) were applied for CHIK virus detection. IgM-indirect immunofluorescent antibody test (IgM-IFA), IgM capture ELISA, and IgG indirect ELISA were applied for detection of serological evidence of CHIK virus infection. Although we could not detect any Chikungunya virus by RT-PCR, 4.1% of the confirmed samples showed high titer for Chikungunya virus by antigen sandwich ELISA. By IgM-IFA, 15.3% were positive for CHIK virus. Thirteen point seven percent showed positive for CHIK virus by IgM-capture ELISA. Eighteen point five percent showed positive for CHIK virus by IgG indirect ELISA.<\/p>\n<\/div>\n\n\n\nKeywords<\/h5>\n\n\n